Key Technical Points of Shaking Culture in Edible Fungi Optimization of flask liquid fermentation medium for xylanase producing strain Pichia pastoris 食用菌液体菌种摇瓶培养操作技术要点毕赤酵母摇瓶产木聚糖酶发酵条件的优化
Its fermentation characteristics was tested in the lab, comparing to the parent strain, its enzymatic activity of shaking culture was higher by26. 在实验室对该菌株进行发酵性能测试,摇瓶酶活力比原菌株提高了26。
Using analytical technic of single factor test statistis, a suitable medium and shaking culture conditions for Penicillium chrysogenum Thom have been selected. 方法:以黄芩苷为指标,采用高效液相色谱法测定黄芩苷含量;采用单因素试验与正交试验优选出黄芩苷的提取工艺。
The optimum fermentation condition included 180 r/ min shaking flask culture at 22 ℃ for 78 h, inoculating amount 8%. 适宜发酵条件:接种量8%,培养温度22℃,摇床转速180r/min。
The mycelia grow better on the static condition than on shaking culture. 鸡(土从)菌菌丝体却需要较低的CO2浓度方能生长良好,它更适宜于摇瓶培养。
The optimum fermentation condition was: first shaking culture for 10 hours, and then static cultivation for 10 days. 适宜的发酵方式为先130r/min振荡培养10h,后静置培养10d。
Eight kinds of culture media were designated to carrying on shaking culture, selecting the YMA, the YMA ammonium phosphate dibasic, the extraction of soil, the defatted soybean leaching solution four kinds of culture medium carried on submerged fermentation culture. 选择8种培养基进行振荡培养,从中选出YMA、YMA磷酸氢二铵、土壤浸出液、黄豆饼浸出液4种培养基进行深层发酵培养。
Under wetted, dried and flooded conditions, the mechanism of pyrite oxidation was studied using column leaching experiment both at 30 ℃ and at natural room temperature and shaking culture experiment at 30 ℃. 采取在湿润、淹水和干燥三种状况于恒温和自然室温条件下用柱子淋洗以及恒温摇床培养试验等方法对黄铁矿氧化机理进行了多方位地研究。
Two cultivation systems, stationary culture ( shake four times every day) and shaking flask culture ( reciprocating shake r), were applied to cultivate Spirulina platensis. 比较两种培养方法:静置培养(每天定时摇动4次)和摇瓶培养(往复式摇床)对钝顶螺旋藻生长的影响。
The contrast experiment of shaking culture and stationary culture, and optimizing culture medium were studied in this paper. 对产L-乳酸的嗜热乳酸杆菌进行了发酵条件的初步研究:摇瓶发酵和静置发酵的对比实验以及培养基的优化实验。
Shaking Flask Culture of B.Thuringiensis 7216 苏云金杆菌7216菌株的摇瓶发酵试验
According to the comparison in the biomass of 8 Volvariella volvacea with shaking flask culture, V_ 93 could produce the highest yield of mycelium. 比较了8株草菇菌种摇瓶培养的菌丝生长量,对适宜液态摇瓶培养高产菌丝的草菇菌株进行筛选,结果表明V93菌株产菌丝体量最高。
Detailed study has been carried out on specific property of the separated efficient bacteria, the chief results are that shaking culture at 110r/ min is favorable for the growth and reproduction of the bacteria; 对分离出的高效菌种的特性进行了较为详细的研究,主要研究结果是:110转/min振荡培养有利于菌种的生长繁殖;
The Affect of Serine Shaking Flask Culture Production by Refilling Sugar 补糖对丝氨酸摇瓶发酵产酸率的影响
Study on shaking flask culture of Mortierella isabellina and extraction of its microbial oils 深黄被孢霉(Mortierellaisabellina)摇瓶培养及其油脂提取研究
Monitoring of dissolved oxygen tension in penicillin shaking flask culture 青霉素摇瓶发酵溶氧浓度的监测
The results showed that the favorable culture conditions were rye liquid medium, pH value 6. 0-7. 0, culture temperature 20-22 C with successive darkening and shaking during culture. The production of crude toxin reached the highest level on the 16th day of culture. 结果表明,适宜马铃薯晚疫病菌粗毒素培养的培养基为黑麦液体培养基,培养基pH值为6.0-7.0,培养温度20-22C,在黑暗条件下连续振荡培养16d时产生的粗毒素的毒性最强。
Study on microbial producing rhamnose by shaking flash culture 微生物发酵生产鼠李糖的摇瓶实验研究
Results showed that the lysostaphin gene could be expressed stably at high level in Bacillus sphaericus and the lysostaphin activity was about 122 u/ ml medium after shaking culture. 经摇瓶发酵试验,溶葡球菌酶的活性约为122u/ml培养液。
The gutta content and productivity in the static solid culture were higher than those in te liquid shaking culture. 固体静止培养的杜仲胶含量和生产效率比液体振荡培养高。
Though examination of shaking bottle aerobic culture, with fusant R2 improve general biology treatment, the bioaugmentation degrading coke-plant waste water was studied. 通过采用摇瓶好氧试验,用融合子R2改善常规生物处理,对焦化废水进行了生物强化降解初步研究。
By this method, we screened 27 soil samples for chitosanase on plates, by comparing the diameters of holos around the colonies and by further shaking culture screening. A strain of bacteria which may secrete higher activity chitosanase was selected and was identified as Serratia ficaria afterwards. 采用这种方法对各地27个土壤样品进行筛选,获得一株细菌,该菌可分泌活力较高的壳聚糖酶。
This study also found that the filter degradation efficiency of flora is much higher under shaking culture conditions than static culture. 研究发现菌群对纤维素的降解效率在振荡培养条件下远高于静置培养条件。